Notice for Polyvalent Antiserum Production from Rabbit
- Option of Adjuvant: For fist shot, Complete Freund’s Adjurant (CFA) should be the choice; as to the following supplementary, Incomplete Freund’s Adjurant (IFA) should be applied or just antigen alone is acceptable.
- Injection Amount
Maximum per local Injection Total Injection Amount ID 0.05-0.1ml 0.5ml SC 0.25-0.5ml 2.5ml IM 0.25-0.5ml 1.0ml
- Immune times and Frequency
- 10 to 45 days between each of immune
- Generally, immune is at least twice; 4 to 8 times is the maximum.
- Blood Collection Amount and Frequency
- Blood that equals to 1% weight is the maximum blood collection amount per week.
- If higher blood collection amount or frequency is required, hematocrit should be under exams weekly as the criteria for the possibility.