Notice for Polyvalent Antiserum Production from Rabbit
Option of Adjuvant: For fist shot, Complete Freund’s Adjurant (CFA) should be the choice; as to the following supplementary, Incomplete Freund’s Adjurant (IFA) should be applied or just antigen alone is acceptable.
Injection Amount
Maximum per local Injection
Total Injection Amount
ID
0.05-0.1ml
0.5ml
SC
0.25-0.5ml
2.5ml
IM
0.25-0.5ml
1.0ml
Immune times and Frequency
10 to 45 days between each of immune
Generally, immune is at least twice; 4 to 8 times is the maximum.
Blood Collection Amount and Frequency
Blood that equals to 1% weight is the maximum blood collection amount per week.
If higher blood collection amount or frequency is required, hematocrit should be under exams weekly as the criteria for the possibility.
